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Cell Signaling Technology Inc primary antibodies against nlrp3

Composite hydrogel promotes the polarization of BV2 cells to M2 types and alleviates PC12 cell pyroptosis in vitro inflammatory environment. (A) Representative western blots showing protein expression of iNOS and Arg-1 in each group, β-actin was utilized as a loading control. (B) Quantitative analysis of relative expression of iNOS and Arg-1. (C) Representative immunofluorescence images of CD68 positive and iNOS positive BV2 cells (scale bar: 20 μm). (D) Representative immunofluorescence images of CD68 positive and Arg-1 positive BV2 cells (scale bar: 20 μm). (E, F) Quantitative analysis of relative fluorescence intensity of iNOS and Arg-1. (G) Representative western blots showing the expression of <t>NLRP3,</t> Caspase-1, IL-1β, ASC, GSDMD-N, and IL-18 protein associated with pyroptosis, β-actin was utilized as a loading control. (H) PI staining of PC12 cells in each group (scale bar, 100 μm). (I) Quantitative analysis of PI staining of PC12 cells (n = 3). (J) Quantitative analysis of relative expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N and IL-18 (n = 3). The data are presented as the means ± SEMs (n = 3); ∗p < 0.05, indicates significant differences; ns, is not significant. Statistical analysis was performed using two-way ANOVA followed by Tukey's multiple comparison test.

Primary Antibodies Against Nlrp3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1658 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 98 stars, based on 1658 article reviews

primary antibodies against nlrp3 - by Bioz Stars, 2026-09

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1) Product Images from "3D-MSCs apoptotic bodies-integrated conductive hydrogel mitigates spinal cord injury via immunoregulation and alleviating neuronal pyroptosis"

Article Title: 3D-MSCs apoptotic bodies-integrated conductive hydrogel mitigates spinal cord injury via immunoregulation and alleviating neuronal pyroptosis

Journal: Bioactive Materials

doi: 10.1016/j.bioactmat.2026.01.043

Figure Legend Snippet: Composite hydrogel promotes the polarization of BV2 cells to M2 types and alleviates PC12 cell pyroptosis in vitro inflammatory environment. (A) Representative western blots showing protein expression of iNOS and Arg-1 in each group, β-actin was utilized as a loading control. (B) Quantitative analysis of relative expression of iNOS and Arg-1. (C) Representative immunofluorescence images of CD68 positive and iNOS positive BV2 cells (scale bar: 20 μm). (D) Representative immunofluorescence images of CD68 positive and Arg-1 positive BV2 cells (scale bar: 20 μm). (E, F) Quantitative analysis of relative fluorescence intensity of iNOS and Arg-1. (G) Representative western blots showing the expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N, and IL-18 protein associated with pyroptosis, β-actin was utilized as a loading control. (H) PI staining of PC12 cells in each group (scale bar, 100 μm). (I) Quantitative analysis of PI staining of PC12 cells (n = 3). (J) Quantitative analysis of relative expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N and IL-18 (n = 3). The data are presented as the means ± SEMs (n = 3); ∗p < 0.05, indicates significant differences; ns, is not significant. Statistical analysis was performed using two-way ANOVA followed by Tukey's multiple comparison test.

Techniques Used: In Vitro, Western Blot, Expressing, Control, Immunofluorescence, Fluorescence, Staining, Comparison

The composite hydrogel inhibits post-SCI pyroptosis in neurons. (A) Immunofluorescence images of residual neurons existing in the anterior horn of the spinal cord (scale bar, 500 μm and 200 μm). (B) Immunofluorescence image of Caspase-1 expression of neurons 3 days after SCI (scale bar, 20 μm). (C) Quantitative analysis of relative fluorescence intensity of Caspase-1 protein expression in neurons within the specified groups (n = 3). (D) Immunofluorescence image of GSDMD-N expression of neurons 3 days after SCI (scale bar, 20 μm). (E) Quantitative analysis of relative fluorescence intensity of GSDMD-N protein expression in neurons within the specified groups (n = 3). (F) Representative western blots showing the expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N, and IL-18 protein 3 days after SCI, GAPDH was utilized as a loading control. (G) Quantitative analysis of relative expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N and IL-18 (n = 3). The data are presented as the means ± SEMs (n = 3); ∗p < 0.05, indicates significant differences; ns, is not significant. Statistical analysis was performed using two-way ANOVA followed by Tukey's multiple comparison test.

Figure Legend Snippet: The composite hydrogel inhibits post-SCI pyroptosis in neurons. (A) Immunofluorescence images of residual neurons existing in the anterior horn of the spinal cord (scale bar, 500 μm and 200 μm). (B) Immunofluorescence image of Caspase-1 expression of neurons 3 days after SCI (scale bar, 20 μm). (C) Quantitative analysis of relative fluorescence intensity of Caspase-1 protein expression in neurons within the specified groups (n = 3). (D) Immunofluorescence image of GSDMD-N expression of neurons 3 days after SCI (scale bar, 20 μm). (E) Quantitative analysis of relative fluorescence intensity of GSDMD-N protein expression in neurons within the specified groups (n = 3). (F) Representative western blots showing the expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N, and IL-18 protein 3 days after SCI, GAPDH was utilized as a loading control. (G) Quantitative analysis of relative expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N and IL-18 (n = 3). The data are presented as the means ± SEMs (n = 3); ∗p < 0.05, indicates significant differences; ns, is not significant. Statistical analysis was performed using two-way ANOVA followed by Tukey's multiple comparison test.

Techniques Used: Immunofluorescence, Expressing, Fluorescence, Western Blot, Control, Comparison

Blocking Assay:

Article Title: Lonicerin alleviates intestinal myenteric neuron injury induced by hypoxia/reoxygenation treated macrophages by downregulating EZH2.
Article Snippet: Funding information This work was supported by the 2022 Dongguan Social Development Science and Technology Project, Grant/Award Number: 20221800905372 Abstract Intestinal ischemia‐reperfusion (IR) injury is a common gastrointestinal disease that induces severe intestinal dysfunction.. Intestinal myenteric neurons participate in maintaining the intestinal function, which will be severely injured by IR.. Macrophages are widely reported to be involved in the pathogenesis of organ IR injury, including intestine, which is activated by NLRP3 signaling.

Incubation:

Article Title: Disulfiram Improves Fat Graft Retention by Modulating Macrophage Polarization With Inhibition of NLRP3 Inflammasome-Mediated Pyroptosis
Article Snippet: Protein concentrations were determined with a BCA (bicinchoninic acid; cat. P0010, Beyotime) kit, and equal amounts of proteins were separated by SDS-PAGE (sodium sodecyl aulfate polyacrylamide gel electrophoresis; cat. P0012A, Beyotime) and transferred to polyvinylidene fluoride membranes. .. The membranes were blocked with 5% bovine serum albumin for 1 hour at room temperature and then incubated overnight at 4°C with primary antibodies against NLRP3 (cat. D4D8T, Cell Signaling Technology, Beverly, MA, 1:1000); pro-caspase-1 and cleaved-caspase-1 (cat. ab179515, Abcam, 1:1000); GSDMD-FL and GSDMD-N (cat. ab219800, Abcam, 1:1000); IL-18 (cat. DF6252, Affinity, 1:1000); IL-1β (cat. AF4006, Affinity, 1:1000); ASC (cat. ab309497, Abcam, 1:1000); and GAPDH (cat. ab9485, Abcam, 1:1000). .. Thereafter, membranes were incubated with horseradish peroxidase–conjugated secondary antibodies for 1 hour at room temperature and signals were detected with an enhanced chemiluminescence kit (cat. 32134, Thermo Fisher, Waltham, MA).

Article Title: Bone marrow mesenchymal stem cell-derived exosomes alleviate DSS-induced inflammatory bowel disease in mice through inhibiting intestinal epithelial cell pyroptosis via delivery of TSG-6
Article Snippet: The membranes were blocked with 5% skimmed milk for 2 h at room temperature and then incubated overnight at 4°C with primary antibodies: anti-CD81 (1:1000, Abcam), anti-CD63 (1:1000, Abcam), anti-TSG101 (1:1000, Abcam), anti-TSG-6 (1:200, Santa Cruz Biotechnology), according to the manufacturer’s instructions. .. Additionally, primary antibodies against NLRP3 (1:1000, Cell Signaling Technology), Caspase-1 (1:1000, Cell Signaling Technology), cleaved Caspase-1 (1:1000, Cell Signaling Technology), Gasdermin D (1:1000, Cell Signaling Technology), ZO-1 (1:1000, Cell Signaling Technology), Occludin (1:1000, Cell Signaling Technology), and GAPDH (1:1000, Cell Signaling Technology) were incubated overnight. ..

Article Title: Butyrate attenuates intestinal inflammation in Crohn’s disease by suppressing pyroptosis of intestinal epithelial cells via the cGSA-STING-NLRP3 axis
Article Snippet: Butyrate can strengthen the intestinal epithelial barrier.. However, the mechanisms by which butyrate affects intestinal epithelial cells (IECs) pyroptosis in Crohn’s disease (CD) remain unclear.. In this study, we collected colonic biopsy samples from CD patients and healthy controls to assess pyroptosis levels.

Article Title: Dexamethasone regulates the SIRT1/NF-κB signaling pathway in neonatal rats with bronchopulmonary dysplasia to counteract NLRP3-inflammasome-induced pyroptosis and oxidative stress.
Article Snippet: Bronchopulmonary dysplasia (BPD) is a lung disease that affects premature infants.. This study explored the effects of dexamethasone (DXM) in BPD and the potential involvement of NLRP3 inflammasome and the SIRT1/ NF-κB pathway in this process.. In vitro, we transfected alveolar epithelial type II (AECII) cells with shRNA SIRT1 and SIRT1 Vector and subjected them to lipopolysaccharide (LPS) and DXM treatments under hyperoxic conditions.

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Journal: Bioactive Materials

Article Title: 3D-MSCs apoptotic bodies-integrated conductive hydrogel mitigates spinal cord injury via immunoregulation and alleviating neuronal pyroptosis

doi: 10.1016/j.bioactmat.2026.01.043

Figure Lengend Snippet: Composite hydrogel promotes the polarization of BV2 cells to M2 types and alleviates PC12 cell pyroptosis in vitro inflammatory environment. (A) Representative western blots showing protein expression of iNOS and Arg-1 in each group, β-actin was utilized as a loading control. (B) Quantitative analysis of relative expression of iNOS and Arg-1. (C) Representative immunofluorescence images of CD68 positive and iNOS positive BV2 cells (scale bar: 20 μm). (D) Representative immunofluorescence images of CD68 positive and Arg-1 positive BV2 cells (scale bar: 20 μm). (E, F) Quantitative analysis of relative fluorescence intensity of iNOS and Arg-1. (G) Representative western blots showing the expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N, and IL-18 protein associated with pyroptosis, β-actin was utilized as a loading control. (H) PI staining of PC12 cells in each group (scale bar, 100 μm). (I) Quantitative analysis of PI staining of PC12 cells (n = 3). (J) Quantitative analysis of relative expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N and IL-18 (n = 3). The data are presented as the means ± SEMs (n = 3); ∗p < 0.05, indicates significant differences; ns, is not significant. Statistical analysis was performed using two-way ANOVA followed by Tukey's multiple comparison test.

Article Snippet: Primary antibodies against NLRP3 (cat. no. 15101) were obtained from Cell Signaling Technology (Beverly, Massachusetts, USA).

Techniques: In Vitro, Western Blot, Expressing, Control, Immunofluorescence, Fluorescence, Staining, Comparison

Journal: Bioactive Materials

Article Title: 3D-MSCs apoptotic bodies-integrated conductive hydrogel mitigates spinal cord injury via immunoregulation and alleviating neuronal pyroptosis

doi: 10.1016/j.bioactmat.2026.01.043

Figure Lengend Snippet: The composite hydrogel inhibits post-SCI pyroptosis in neurons. (A) Immunofluorescence images of residual neurons existing in the anterior horn of the spinal cord (scale bar, 500 μm and 200 μm). (B) Immunofluorescence image of Caspase-1 expression of neurons 3 days after SCI (scale bar, 20 μm). (C) Quantitative analysis of relative fluorescence intensity of Caspase-1 protein expression in neurons within the specified groups (n = 3). (D) Immunofluorescence image of GSDMD-N expression of neurons 3 days after SCI (scale bar, 20 μm). (E) Quantitative analysis of relative fluorescence intensity of GSDMD-N protein expression in neurons within the specified groups (n = 3). (F) Representative western blots showing the expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N, and IL-18 protein 3 days after SCI, GAPDH was utilized as a loading control. (G) Quantitative analysis of relative expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N and IL-18 (n = 3). The data are presented as the means ± SEMs (n = 3); ∗p < 0.05, indicates significant differences; ns, is not significant. Statistical analysis was performed using two-way ANOVA followed by Tukey's multiple comparison test.

Article Snippet: Primary antibodies against NLRP3 (cat. no. 15101) were obtained from Cell Signaling Technology (Beverly, Massachusetts, USA).

Techniques: Immunofluorescence, Expressing, Fluorescence, Western Blot, Control, Comparison

Regulation of NLRP3 inflammasome activation by antibiotic‐loaded bone cement (ALBC). (a) Schematic diagram of NLRP3 inflammasome activation; (b) immunohistochemistry detection of NLRP3 and Caspase‐1 expression in full‐thickness skin tissue surrounding the wound edge in mice; (c, d) western blot analysis of Pro‐Caspase‐1 and cleaved Caspase‐1 expression in cells; (e) IF detection of NLRP3 and apoptosis‐associated speck‐like protein containing a CARD (ASC) co‐localization. N = 6; cell experiments were repeated three times; *** p < 0.001 between groups. ALBC‐H, high‐dose ALBC; ALBC‐L, low‐dose ALBC; ALBC‐M, medium‐dose ALBC; HG, high‐glucose.

Journal: Bioengineering & Translational Medicine

Article Title: Engineering macrophage phenotype switching via nucleotide‐binding oligomerization domain‐like receptor protein 3 inflammasome inhibition: A translational approach using antibiotic cement for diabetic foot ulcers

doi: 10.1002/btm2.70073

Figure Lengend Snippet: Regulation of NLRP3 inflammasome activation by antibiotic‐loaded bone cement (ALBC). (a) Schematic diagram of NLRP3 inflammasome activation; (b) immunohistochemistry detection of NLRP3 and Caspase‐1 expression in full‐thickness skin tissue surrounding the wound edge in mice; (c, d) western blot analysis of Pro‐Caspase‐1 and cleaved Caspase‐1 expression in cells; (e) IF detection of NLRP3 and apoptosis‐associated speck‐like protein containing a CARD (ASC) co‐localization. N = 6; cell experiments were repeated three times; *** p < 0.001 between groups. ALBC‐H, high‐dose ALBC; ALBC‐L, low‐dose ALBC; ALBC‐M, medium‐dose ALBC; HG, high‐glucose.

Article Snippet: Primary antibodies against NLRP3 (CSB‐ PA209407 , 1:100, Cusabio) and Caspase‐1 (CSB‐ PA001227 , 1:100, Cusabio) were diluted according to the manufacturer's instructions, applied to the slides, and incubated overnight at 4°C.

Techniques: Activation Assay, Immunohistochemistry, Expressing, Western Blot

Role of the NLRP3 inflammasome in the therapeutic effect of antibiotic‐loaded bone cement (ALBC) in diabetic foot ulcer (DFU) mice. (a) Schematic diagram of the treatment protocol in which diabetic foot ulcer (DFU0 mice received high‐dose antibiotic‐loaded bone cement [ALBC‐H] in combination with the NLRP3 activator Nigericin or the inhibitor MCC950); (b) wound healing rates on Days 7 and 14 in each group; (c) immunohistochemistry analysis of NLRP3 and Caspase‐1 expression in skin tissues from each group; (d) enzyme‐linked immunosorbent assay analysis of IL‐1β and IL‐18 cytokine levels; (e) immunofluorescence detection of TNF‐α, iNOS, CD206, and CD163 protein expression in mouse skin tissues. N = 6; * p < 0.05, ** p < 0.01, *** p < 0.001 between groups.

Journal: Bioengineering & Translational Medicine

doi: 10.1002/btm2.70073

Figure Lengend Snippet: Role of the NLRP3 inflammasome in the therapeutic effect of antibiotic‐loaded bone cement (ALBC) in diabetic foot ulcer (DFU) mice. (a) Schematic diagram of the treatment protocol in which diabetic foot ulcer (DFU0 mice received high‐dose antibiotic‐loaded bone cement [ALBC‐H] in combination with the NLRP3 activator Nigericin or the inhibitor MCC950); (b) wound healing rates on Days 7 and 14 in each group; (c) immunohistochemistry analysis of NLRP3 and Caspase‐1 expression in skin tissues from each group; (d) enzyme‐linked immunosorbent assay analysis of IL‐1β and IL‐18 cytokine levels; (e) immunofluorescence detection of TNF‐α, iNOS, CD206, and CD163 protein expression in mouse skin tissues. N = 6; * p < 0.05, ** p < 0.01, *** p < 0.001 between groups.

Techniques: Immunohistochemistry, Expressing, Enzyme-linked Immunosorbent Assay, Immunofluorescence

Effects of antibiotic‐loaded bone cement on wound tissues of diabetic foot ulcer (DFU) patients. (a) Immunofluorescence detection of M1 and M2 macrophage phenotype markers in DFU patient wound tissues; (b) immunohistochemistry analysis of NLRP3 and Caspase‐1 expression in DFU patient wound tissues. N = 3; *** p < 0.001 between groups.

Journal: Bioengineering & Translational Medicine

doi: 10.1002/btm2.70073

Figure Lengend Snippet: Effects of antibiotic‐loaded bone cement on wound tissues of diabetic foot ulcer (DFU) patients. (a) Immunofluorescence detection of M1 and M2 macrophage phenotype markers in DFU patient wound tissues; (b) immunohistochemistry analysis of NLRP3 and Caspase‐1 expression in DFU patient wound tissues. N = 3; *** p < 0.001 between groups.

Techniques: Immunofluorescence, Immunohistochemistry, Expressing

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primary antibodies against nlrp3 (Cell Signaling Technology Inc)

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